Hu,
Mu,
Rt,
Po,
Bv,
Ca,
Ch,
Gp,
Ha,
Pm,
Rb,
ShSpecies Glossary
Reactivity: Hu, Mu, Rt, Po, Bv, Ca, Ch, Gp, Ha, Pm, Rb, Sh
WB, Simple Western, ELISA, Flow, ICC/IF, IHC, IP, MS
Applications: WB, Simple Western, ELISA, Flow, ICC/IF, IHC, IP, MS
Host: Rat Monoclonal
Sample Size Available
Sizes: Sample Size Available
![Western Blot: HSPA8/HSC71/Hsc70 Antibody (1B5) [NBP1-97868] - Analysis of HSC70/HSP73: Lane 1: MW marker, Lane 2: HSC70/HSP73 (bovine), (recombinant) Lane 3: HSP70/HSP72 (human), (recombinant) Lane 4: 3T3, (Cell Lysate) Lane 5: PC-12, (Cell Lysate), Lane 6: CHO-K1 Cell Lysate, Lane 7: GPC-16 Cell Lysate, Lane 8: HeLa, (Cell Lysate).](http://images.novusbio.com/fullsize/HSPA8-HSC71-Hsc70-Antibody-1B5-Western-Blot-NBP1-97868-img0002.jpg "Western Blot: HSPA8/HSC71/Hsc70 Antibody (1B5) [NBP1-97868] - Analysis of HSC70/HSP73: Lane 1: MW marker, Lane 2: HSC70/HSP73 (bovine), (recombinant) Lane 3: HSP70/HSP72 (human), (recombinant) Lane 4: 3T3, (Cell Lysate) Lane 5: PC-12, (Cell Lysate), Lane 6: CHO-K1 Cell Lysate, Lane 7: GPC-16 Cell Lysate, Lane 8: HeLa, (Cell Lysate).")
![Immunohistochemistry-Paraffin: HSPA8/HSC71/Hsc70 Antibody (1B5) [NBP1-97868] - Analysis of human spleen tissue stained with HSC70/HSP73, mAb (1B5) at 10ug/ml.](http://images.novusbio.com/fullsize/HSPA8-HSC71-Hsc70-Antibody-1B5-Immunohistochemistry-Paraffin-NBP1-97868-img0001.jpg)
![Western Blot: HSPA8/HSC71/Hsc70 Antibody (1F2-H5) [NBP2-12880] - Western Blot analysis of Human Cell lysates showing detection of HSPA8/HSC71/Hsc70 protein using Mouse Anti-HSPA8/HSC71/Hsc70 Monoclonal Antibody, Clone 1F2-H5 (NBP2-12880). Load: 15 ug. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-HSPA8/HSC71/Hsc70 Monoclonal Antibody (NBP2-12880) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT. 1: mix of 10 different human cell lines, 2: Hsp72 recombinant protein, and 3: Hsc70(Hsp73) recombinant protein.](http://images.novusbio.com/fullsize/HSPA8-HSC71-Hsc70-Antibody-1F2-H5-Western-Blot-NBP2-12880-img0010.jpg "Western Blot: HSPA8/HSC71/Hsc70 Antibody (1F2-H5) [NBP2-12880] - Western Blot analysis of Human Cell lysates showing detection of HSPA8/HSC71/Hsc70 protein using Mouse Anti-HSPA8/HSC71/Hsc70 Monoclonal Antibody, Clone 1F2-H5 (NBP2-12880). Load: 15 ug. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-HSPA8/HSC71/Hsc70 Monoclonal Antibody (NBP2-12880) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT. 1: mix of 10 different human cell lines, 2: Hsp72 recombinant protein, and 3: Hsc70(Hsp73) recombinant protein.")
![Immunocytochemistry/Immunofluorescence: HSPA8/HSC71/Hsc70 Antibody (1F2-H5) [NBP2-12880] - Analysis using Mouse Anti-Hsc70 Monoclonal Antibody, Clone 1F2-H5. Tissue: HaCaT cells. Species: Human. Fixation: Cold 100% methanol for 10 minutes at -20 degrees C. Primary Antibody: Mouse Anti-Hsc70 Monoclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Bright cytoplasmic staining, duller nuclear staining.](http://images.novusbio.com/fullsize/HSPA8-HSC71-Hsc70-Antibody-1F2-H5-Immunocytochemistry-Immunofluorescence-NBP2-12880-img0009.jpg)
![Immunocytochemistry/Immunofluorescence: HSPA8/HSC71/Hsc70 Antibody (1F2-H5) [NBP2-12880] - Analysis using Mouse Anti-Hsc70 (Hsp73) Monoclonal Antibody, Clone 1F2-H5. Tissue: Heat Shocked HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Mouse Anti-Hsc70 (Hsp73) Monoclonal Antibody at 1:100 for 12 hours at 4 degrees C. Secondary Antibody: R-PE Goat Anti-Mouse (yellow) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Cytoplasm. Melanosome. Localizes to nucleus upon heat shock. Magnification: 20x. (A) DAPI (blue) nuclear stain. (B) Anti-Hsc70 (Hsp73) Antibody. (C) Composite.](http://images.novusbio.com/fullsize/HSPA8-HSC71-Hsc70-Antibody-1F2-H5-Immunocytochemistry-Immunofluorescence-NBP2-12880-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: HSPA8/HSC71/Hsc70 Antibody (1F2-H5) [NBP2-12880] - Analysis using Mouse Anti-Hsc70 (Hsp73) Monoclonal Antibody, Clone 1F2-H5. Tissue: Heat Shocked HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Mouse Anti-Hsc70 (Hsp73) Monoclonal Antibody at 1:100 for 12 hours at 4 degrees C. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Cytoplasm. Melanosome. Localizes to nucleus upon heat shock. Magnification: 100x. (A) DAPI (blue) nuclear stain. (B) Anti-Hsc70 (Hsp73) Antibody. (C) Composite.](http://images.novusbio.com/fullsize/HSPA8-HSC71-Hsc70-Antibody-1F2-H5-Immunocytochemistry-Immunofluorescence-NBP2-12880-img0007.jpg)
![Western Blot: HSC70 Antibody (plant) [NBP1-97498] - Analysis of HSC70 (plant, ER, BiP): Lane 1: MW marker, Lane 2: Apple, Lane 3: Carrot, Lane 4: Spinach.](http://images.novusbio.com/fullsize/HSC70-Antibody-plant-Western-Blot-NBP1-97498-img0001.jpg "Western Blot: HSC70 Antibody (plant) [NBP1-97498] - Analysis of HSC70 (plant, ER, BiP): Lane 1: MW marker, Lane 2: Apple, Lane 3: Carrot, Lane 4: Spinach.")
![Western Blot: HSC70 Antibody (5B7) [NBP1-97454] - Analysis of HSC70: Lane 1: MW marker, Lane 2: DnaK Recombinant E. coli Protein, Lane 3: Banana Lysate, Lane 4: Orange Lysate, Lane 5: Carrot Lysate, Lane 6: Cytosolic wheat HSC70.](http://images.novusbio.com/fullsize/HSC70-Antibody-5B7-Western-Blot-NBP1-97454-img0001.jpg "Western Blot: HSC70 Antibody (5B7) [NBP1-97454] - Analysis of HSC70: Lane 1: MW marker, Lane 2: DnaK Recombinant E. coli Protein, Lane 3: Banana Lysate, Lane 4: Orange Lysate, Lane 5: Carrot Lysate, Lane 6: Cytosolic wheat HSC70.")